Kilwinning Rangers, who last won the trophy in 1999, will be at home to Beith, who landed the top honour in 2016.
The hotly anticipated clash is scheduled for Saturday, September 29.
Irvine Vics will host Kirkintilloch Rob Roy and Irvine Meadow will be favoured to come through away to St Anthony's.
There will be another Ayrshire derby as Annbank host League Two rivals Ardeer Thistle.
Girvan host Culter and Whitletts Vics host Lanark.
Dalry Thistle are away to Lochee United.
Former winners Kilbirnie Ladeside are at Gartcairn and holders Auchinleck Talbot have home advantage against Lochee Harp.
The full draw for the Macron Scottish Junior Cup, affecting Ayrshire teams is, as follows:
Kinnoull v Saltcoats Victoria
Troon v Lochee Harp
Auchinleck Talbot v Forfar West End
Cumnock v Coupar Angus
Cumbernauld United v Glenafton Athletic
Girvan v Culter
Muirkirk v Yoker Athletic
Gartcairn v Kilbirnie Ladeside
Bridge of Don Thistle v Ardrossan Winton Rovers
Maybole v Aberdeen University
Kilwinning Rangers v Beith
Hurlford United v Dufftown
Lochee United v Dalry Thistle
St Anthony's v Irvine Meadow
Irvine Victoria v Kirkintilloch Rob Roy
Petershill v Darvel
Royal Albert v Lugar Boswell Thistle
Annbank United v Ardeer Thistle
Arbroath Victoria v Largs Thistle
Bathgate Thistle v Craigmark Burntonians
Kello Rovers v Blairgowrie
Whitletts Victoria v Lanark United
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